Trying to create a test SSH connection to my node

Trying to create a test SSH connection to my node. Connect using key-based authentication, fails.

PS C:\Users\Jeffrey\learn-chef\cookbooks> ssh -i \Users\Jeffrey\aws\awsjmr.pem ec2-user@ec2-35-167-125-177.us-west-2.compute.amazonaws.com
Permission denied (publickey,gssapi-keyex,gssapi-with-mic).
PS C:\Users\Jeffrey\learn-chef\cookbooks>

Not sure what the problem is, any suggestions?
Thank you,
Jeffrey